NeuroMab™ Anti-CXCR4 BBB Shuttle Antibody(NRZP-1022-ZP3805)

Figure 1 is a series of FACS analysis graphs showing that CXCR4 liposomes can bind the conformation-sensitive anti-CXCR4 monoclonal antibody 12G5 In addition, FACS analysis also showed that CXCR4 proteoliposomes were able to bind to the CXCR4 ligand SDF-1.

Figure 2 shows the characterization results of CXCR4 paramagnetic liposomes (CXCR4-PMPLs)

CXCR4-PMPL (lower panel) was stained with CXCR4 conformation-dependent mAb 12G5-PE or its natural ligand SDF-1-Fc fusion protein, followed by FITC-anti-mouse IgG. CCR5-PMPL (upper panel) and IgG2a-PE and CCR5-specific mAb 2D7-PE were used as negative controls.

Figure 3 is a histogram showing the downregulation of cell surface expressed CXCR4 protein.

Figure 4 is a series of FACS analysis graphs showing that X33, X48, X18, X19 and X20 specifically bind to CXCR4 cells.

Figure 5 is a graph of FACS analysis demonstrating that X18, X19 and X20 (except X33 and X48) compete with 12G5 for binding to CXCR4.

Figure 6 is a graph of FACS scan analysis showing that scFv clones 33 and 48 compete with neutralizing anti-CXCR4 monoclonal antibody 12G5 for binding to CXCR4.

Figure 7 is a series of graphs demonstrating inhibition of HIV-1 reporter virus entry into Cf2ThCD4CXCR4 cells.

Figure 8 is a series of graphs showing inhibition of HIV-1 reporter virus entry by scFvs 33 and 48.

Figure 9 is a graph showing the effect of scFvs 33 and 48 on SDF-1 mediated chemotaxis of Jurkat T cells.