Product
NeuroMab™ Mouse Anti-THETM beta Actin Monoclonal Antibody (CBP2124)
[CAT#: NAB-2011-ZP10]
- Host Species:
- Mouse
- Species Reactivity:
- Mouse; Rabbit; Chicken
- Applications:
- ELISA; WB
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
β-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to β-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Immunogen
A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Species Reactivity
Mouse; Rabbit; Chicken
Clonality
Monoclonal
Host Species
Mouse
Isotype
IgG2a, k
Clone Number
CBP2124
Applications
ELISA; WB
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other Applications: user-optimized
The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other Applications: user-optimized
Conjugation
Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1609
Product Properties
Concentration
40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. Note: 100 µg, 200µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA
Purification
Protein A or G affinity column
Endotoxin Level
Low Endotoxin
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Handling Advice
Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Data
Publications
Publications (0)
Neural Antibodies
For Research Use Only. Not For Clinical Use.
