NeuroMab™ Mouse Anti-THETM beta Actin Monoclonal Antibody (CBP2124)

Product Overview

Description

β-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to β-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.

Immunogen

A synthetic peptide DDDIAALVVDNGSG coupled to KLH

Species Reactivity

Mouse; Rabbit; Chicken

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a, k

Clone Number

CBP2124

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.1-1.0 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1609

Product Properties

Concentration

40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. Note: 100 µg, 200µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA

Purification

Protein A or G affinity column

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.