NeuroMab™ Rat Anti-β-actin Monoclonal Antibody (CBP2464)

Product Overview

Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Immunogen

Human ACTB peptide (1-13 a.a.).

Species Reactivity

Human; Mouse; Rat; Zebrafish; Drosophila

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a, κ

Clone Number

CBP2464

Applications

Application Notes

WB (0.001-0.2 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1803

Product Properties

Form

Liquid

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Handling Advice

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

β-actin

Official Name

ACTB

Full Name

Beta-actin

Alternative Names

ACTB; BRWS1; PS1TP5BP1; Beta-actin; actin; beta; actin beta

Gene ID

60 (Human); 11461 (Mouse)

Uniprot ID

P60709 (Human); P60710 (Mouse)

References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Publications