Product
Rabbit Anti-Beta Actin Monoclonal Antibody (CBP2130)
[CAT#: NAB-2011-ZP37]
- Host Species:
- Rabbit
- Species Reactivity:
- Rabbit
- Applications:
- ELISA; WB
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
Beta-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to β-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Beta-Actin Antibody, mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with a synthetic peptide coupled to KLH.
Beta-Actin Antibody, mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with a synthetic peptide coupled to KLH.
Immunogen
A synthetic peptide coupled to KLH
Species Reactivity
Rabbit
Clonality
Monoclonal
Host Species
Rabbit
Isotype
IgG
Clone Number
CBP2130
Applications
ELISA; WB
Application Notes
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by primary/secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
Western Blotting: 1:1,000-1:5,000
ELISA: 1:5,000-1:10,000
Other Applications: user-optimized
Optimal working dilutions must be determined by the end user.
The following concentration ranges are recommended starting points for this product.
Western Blotting: 1:1,000-1:5,000
ELISA: 1:5,000-1:10,000
Other Applications: user-optimized
Optimal working dilutions must be determined by the end user.
Conjugation
Unconjugated
Product Properties
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It could be customized for bulk order.)
Purification
Protein A affinity column
Shipping
The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Storage
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Handling Advice
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Data
Publications
Publications (0)
Neural Antibodies
For Research Use Only. Not For Clinical Use.
