NeuroMab™ Mouse Anti-Human Dystroglycan Monoclonal Antibody (CBP2226)

Product Overview

Description

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and utrophin scaffolds axin to the cytoskeleton. β-dystroglycan has also been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.

Immunogen

KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b, κ

Clone Number

CBP2226

Applications

WB; IHC-P; IP

Application Notes

WB (1.0 -5.0 μg/mL); IHC-P (0.1-10 μg/ml)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1652

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

DAG1

Official Name

DAG1

Full Name

Alpha Dystroglycan

Alternative Names

DAG1; 156DAG; A3a; AGRNR; DAG; MDDGC7; MDDGC9; MDDGA9; dystroglycan 1; LGMDR16

Gene ID

1605 (Human); 13138 (Mouse); 114489 (Rat)

Uniprot ID

Q14118 (Human); Q62165 (Mouse)

References

1. Murphy S and Ohlendieck K. 2015. Comput. Struct. Biotechnol. J. Nov 26;14:20-7.
2. Moore CJ and Winder SJ. 2012. Neuromuscul. Disord. Nov: 22(11): 959.

Publications