NeuroMab™ Mouse Anti-Human CDCP1 Monoclonal Antibody (CBP2297)

Description

The CUB1 antibody recognizes CD318 also known as CDCP1, CUB domain containing protein 1, and SIMA135. CD318 is a transmembrane protein containing three CUB domains with a predicted molecular weight of approximately 93 kD. Alternately spliced isoforms have also been reported. CD318 is a marker for immature hematopoietic progenitor cell subsets and is also expressed in mesenchymal stem cells and neural progenitor cell populations. This marker is also widely distributed in a variety of cell types including keratinocytes, skeletal muscle, colon, kidney, lung, small intestine. CD318 is overexpressed in colon and lung tumors and expression level has been shown to correlate with metastatic ability of carcinomas. CD318 interacts with plasminogen, matriptase, and c-Src (phosphorylates residue Tyr734). The function of CD318 is not well understood although it is thought to be involved in early hematopoiesis. The CUB1 antibody has been shown to be useful for flow cytometry.

Immunogen

NIH-3T3 cells transfected with human CDCP1

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b, κ

Clone Number

CBP2297

Applications

FC

Application Notes

FCM (≤0.125 µg per million cells in 100 µl volume)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1692

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CDCP1

Official Name

CDCP1

Full Name

CUB Domain Containing Protein 1

Alternative Names

CUB Domain Containing Protein 1; Subtractive Immunization M Plus HEp3-Associated 135 KDa Protein; Transmembrane And Associated With Src Kinases; Membrane Glycoprotein Gp140; SIMA135; TRASK; CUB Domain-Containing Protein 1; CD318 Antigen; CD318;

Gene ID

64886 (Human); 64866 (Mouse)

Uniprot ID

Q9H5V8 (Human); Q5U462 (Mouse)

1. Brown TA, et al. 2004. J. Biol. Chem. 279:14772.
2. Conze T, et al. 2003. Ann. N.Y. Acad. Sci. 996:222.
3. Hooper JD, et al. 2003. Oncogene 22:1783.