NeuroMab™ Mouse Anti-ATG5 Monoclonal Antibody (CBP2478)

Product Overview

Description

Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2478

Applications

ICC

Application Notes

ICC (1.0-5.0 μg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1814

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

ATG5

Official Name

ATG5

Full Name

Autophagy related 5

Alternative Names

ATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5; autophagy related 5; SCAR25

Gene ID

9474 (Human); 11793 (Mouse)

Uniprot ID

Q9H1Y0 (Human); Q99J83 (Mouse)

Protocols

Its related pathways are Macroautophagy and Shigellosis.

References

1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed

Publications