CCK1 Cholecystokinin Receptor Assay Cell Line

[CAT#: NCL2110P298]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: CCK1 (CCKAR)/U2OS
Official Full Name: Cholecystokinin A receptor
DNA Accession Number: GenBank: AY322549
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: G418
Size:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The HiTector CCK1 contains U2OS cells stably expressing unlabeled human cholecystokinin A receptor (CCK1). The Creative Biolabs HiTector CCK1 cell line is designed to test compounds or analyze their ability to modulate cholecystokinin A receptors. When the agonist binds to CCK1, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (calcium). This cell line has been validated by measuring the increase in calcium in the cytosol. The high reproducibility of this assay allows monitoring of the CCK1 activation process in high-throughput screening.

Cell Types

Other Cells

Research Areas

GPCR

Assay

CCK1 Cholecystokinin Receptor Assay

Assay Description

The CCK1 Cholecystokinin Receptor Assay from Creative Biolabs allows the detection of compounds, or analysis of their ability to modulate CCKAR. When the ligand binds to CCKAR, it activates the G protein; this in turn triggers a calcium-mediated cellular response. Binding CCK to CCKAR induce intracellular calcium mobilization and PKC activation through PLC-IP3 and DAG pathways, respectively.

CCK1 Cholecystokinin Receptor Assay includes the measurement of calcium concentration in the cytoplasm. The high reproducibility of this assay allows monitoring of the CCKAR receptor activation process in high-throughput screening.

Assay Characteristics

Readout: Calcium Flux

Agonist: CCK Octapeptide

EC50 Agonist: 4.17 x 10⁶-10 M for Calcium Flux

Type of Assay: Cell-based

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Target Details

Target

CCKA

Official Name

CCKA

Full Name

CCK1 receptor

Alternative Names

CCK1 receptor; CCK-A; CCKA; Cck-ar; CCK-A receptor; CCK1-R; cholecystokinin A receptor; cholecystokinin receptor type A; cholecystokinin-1 receptor; AW106902

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