ETB Endothelin Receptor Assay Cell Line

[CAT#: NCL2110P290]

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

Cell line information: ENDRB Multiplexed Maxensor cell line
Official Full Name: Endothelin B receptor
DNA Accession Number: AY275463
Host Cell: U2OS
Resistance: G418 + Puromycin + Hygromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The Maxensor ENDRB contains U2OS cells stably expressing cAMP Maxensor-FP650 biosensor, calcium Maxensor-tGFP biosensor and endothelin receptor B (unlabeled). The Creative Biolabs Maxensor ENDRB cell line is designed to test compounds or analyze their ability to modulate endothelin receptor B. When the agonist binds to ENDRB, the G protein is activated, which in turn triggers a cellular response mediated by cAMP and calcium. This cell line has been verified by analyzing the strength and intracellular distribution of cAMP and calcium biosensor by measuring the increase of cAMP and calcium in the cytosol. Using endothelin as an agonist and measuring by fluorescence intensity and image analysis, this highly reproducible measurement has been verified.

Cell Types

Other Cells

Application Notes

About the Maxensor biosensor series:
The Maxensor biosensor series is based on fluorescent peptides, which can measure the fluctuations of calcium and inhibitory protein signaling pathways, and change their intracellular localization and fluorescence intensity emission. Before the stimulation mediated by the agonist of interest, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the Maxensor biosensor, which promotes cell relocation and fluorescence increase in the vesicle transport of cells. In cell lines co-expressing Maxensor biosensor and GPCR, the activity of living cells can be easily quantified by image analysis or fluorescence emission in a microplate reader.

Research Areas

GPCR

Assay

ETB Endothelin Receptor Assay

Assay Description

The ETB Endothelin Receptor Assay from Creative Biolabs allows compounds to be tested or analyzed for their ability to modulate EDNRB. When the ligand binds to EDNRB, it activates the G protein, which in turn triggers a cellular response that contains calcium and cAMP. Our EDNRB assay uses endothelin I as a positive control and measures the changes in cAMP and calcium concentrations in the cytoplasm. The high reproducibility of this assay allows monitoring of the EDNRB activation process in high-throughput screening.

Assay Characteristics

Readout: Calcium Flux & cAMP Flux

Agonist: Endothelin I

EC50 Agonist: 2.38 x 10⁶-9 M for Calcium Assay & 5.61 x 10⁶-9 for cAMP flux

Type of Assay: Cell-based/Functional

Detection Method: Fluorimetry for both, Calcium and cAMP

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.