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Creative Biolabs

Human ATG3 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP519]

Human ATG3 knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human ATG3 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurodegeneration

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

ATG3

Official Name

ATG3

Full Name

Autophagy Related 3

Alternative Names

Autophagy Related 3; Autophagy-Related Protein 3; APG3-LIKE; APG3L; HApg3; APG3; ATG3 Autophagy Related 3 Homolog (S. Cerevisiae); Ubiquitin-Like-Conjugating Enzyme ATG3;

Gene ID

64422 (Human); 67841 (Mouse); 171415 (Rat)

Uniprot ID

Q9NT62 (Human); Q9CPX6 (Mouse); Q6AZ50 (Rat)
Publications

Publications (0)

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