Human CHO-K1/H3/Gα15 Stable Cell Line

[CAT#: NCL20120074CR]

H3 Histamine Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

The histamine receptor H3 is a Gi-coupled GPCR expressed in the thalamus, caudate nucleus, putamen, cerebellum, amygdala, substantia nigra, hippocampus, hypothalamus and cerebral cortex. Activation of H3 receptor in human nasal mucosa inhibits sympathetic vasoconstriction. Expression of human H3 receptor splice variants in CHO cells shows that a deletion in the 2nd transmembrane domain alters ligand binding and deletions in the 3rd intracellular loop result in signal transduction being abolished.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

H3/HRH3

Family

Histamine receptor

Strain

H3/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

HRH3

Official Name

HRH3

Alternative Names

H3 receptor; GPCR97; MGC105370; H3R; HH3R; histamine H3 receptor; AW049250

Gene ID

11255(Human); 99296(Mouse); 85268(Rat)

Uniprot ID

Q9Y5N1(Human); P58406(Mouse); Q9QYN8(Rat)

References

1. Montagna, E., Crux, S., Luckner, M., Herber, J., Colombo, A. V., Marinković, P.,.. & Sgobio, C. (2019). In vivo Ca2+ imaging of astrocytic microdomains reveals a critical role of the amyloid precursor protein for mitochondria. Glia, 67(5), 985-998. 2. Xu, Y., Wu, X., Wang, S., Yang, C., Li, Y., & Cao, Y. (2019). Hydroxyapatite Nanoparticle-Crosslinked Peptide Hydrogels for Three-Dimensional Culture and Differentiation of MC3T3-E1 Osteoblasts. Journal of biomedical nanotechnology, 15(12), 2351-2362. 3. Lan, J., Rollins, J. A., Zang, X., Wu, D., Zou, L., Wang, Z.,.. & Chen, D. (2019). Translational regulation of non-autonomous mitochondrial stress response promotes longevity. Cell reports, 28(4), 1050-1062.

Publications