Human HEK293/AT1 Stable Cell Line

[CAT#: NCL20120025CR]

Angiotensin Receptor 1 Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

α1-ARs belong to the G protein-coupled receptor family and consist of single polypeptide chains predicted to have seven transmembrane spanning domains. With similar pharmacological and signaling properties, α1-AR subtypes act through Gq/11 proteins to activate phospholipase C, increase both inositol 1,4,5-trisphosphate production and intracellular Ca2+. Once activated by binding, α1-ARs initiate the cellular pathways leading to the regulation of physiological effects, including blood pressure maintenance, glucose metabolism, renal sodium reabsorption, and cardiac inotropy.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR; Neural Signal Transduction

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

DMEM, 10% FBS, 300 μg/ml G418

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

AT1

Family

Angiotensin receptor

Strain

AT1 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

AT1

Official Name

AT1

Alternative Names

AGTR1B; AT2R1; AT2R1B; angiotensin II receptor type 1; AII-I; AII alpha; angiotensin receptor 1B; AT1R; angiotensin II type-1 receptor; angiotensin II type-1B receptor; angiotensin receptor 1; AT1A; AT1R; Angiotensin II receptor type 1 (AT1A); angiotensin II receptor; type 1a; angiotensin II type-1A receptor; vascular type-1 angiotensin II receptor; Agtr-1b; Angtr-1b;

Gene ID

9197(Human); 11416(Mouse); 64018(Rat)

Uniprot ID

O00400(Human); Q99J27(Mouse); Q6AYY8(Rat)

References

1. Montagna, E., Crux, S., Luckner, M., Herber, J., Colombo, A. V., Marinković, P.,.. & Sgobio, C. (2019). In vivo Ca2+ imaging of astrocytic microdomains reveals a critical role of the amyloid precursor protein for mitochondria. Glia, 67(5), 985-998. 2. Xu, Y., Wu, X., Wang, S., Yang, C., Li, Y., & Cao, Y. (2019). Hydroxyapatite Nanoparticle-Crosslinked Peptide Hydrogels for Three-Dimensional Culture and Differentiation of MC3T3-E1 Osteoblasts. Journal of biomedical nanotechnology, 15(12), 2351-2362. 3. Lan, J., Rollins, J. A., Zang, X., Wu, D., Zou, L., Wang, Z.,.. & Chen, D. (2019). Translational regulation of non-autonomous mitochondrial stress response promotes longevity. Cell reports, 28(4), 1050-1062.

Publications