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Creative Biolabs

Human PGR Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP445]

Human PGR knockout cell lysate

Species:
Human
Cell Types:
Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human PGR has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 4 bp deletion in exon5 and 91 bp insertion in exon5.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neuroendocrinology

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

PGR

Official Name

PGR

Full Name

progesterone receptor

Alternative Names

PGR; NR3C3; PR; progesterone receptor

Gene ID

8993(Human); 84387(Rat)

Uniprot ID

O75594(Human); Q9JLN4(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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