iNeu™ Plated Human Cerebral Cortical Neurons - Alzheimer's Disease Patient (PSEN1 M146L)

[CAT#: NCL-2101-ZP27]

Species:: Human

Cell Types:: Cortical Neurons

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Product Overview

Description

Creative Biolabs human iPSC-derived cerebral cortical neurons express typical markers of cerebral cortical neurons, such as TUJ1, TBR1, CTIP2, BRN2 and CUX1. They are electrically active and can form functional synapses and neuronal networks in vitro.

Creative Biolabs provides pre-plated neurons that differentiate synchronously with neural stem cells using our fully defined neuron differentiation method. Using our special coating reagent and Neural Maintenance-XF medium, iPSC-derived neurons can be cultured for a long time.

Our expert scientists perform neuron differentiation and supply neurons in a 96-well plate format (with 60 fillings in the center). Electroplated neurons can be measured at any time, saving you time and resources. The iPSC-derived neurons are shipped live so the neurons are not stressed by cryopreservation.

Neurological Disease Models

AD-related Cells

Cell Types

Cortical Neurons

Application Notes

Please be aware that the correct volume of Neural Maintenance-XF Medium (Cat. NCC-2101-ZP06) needed for your specific order volume of cells will be supplied.

Relevant Diseases

Alzheimer's Disease

Species

Human

Properties

Size

Center 60 wells of 96-well plate (Order here)

Tissue Source

Fibroblasts (53 Yr Male)

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.

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