iNeu™Vol™ Cationic Voltage Biosensor Assay using Human Cortical Neurons

[CAT#: NCL-2103-P54]

Species:: Human

Cell Types:: Cortical Neurons

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Product Overview

Description

iNeuVol™: cationic voltage biosensor assay using human iPS-derived. They are derived from a completely defined proprietary neural induction and maturation condition without integration of induced pluripotent stem cell (iPSC) lines. When plated and cultured in a monolayer, iNeuVol™ cells have a polarized structure and express markers such as MAP2 and β-Tuj. We have verified that iNeuVol™ cells are cation-conducting neurons when exposed to solutions with fluctuating concentrations of sodium, calcium, potassium, magnesium and proton/H+. Use a proprietary method to introduce iNeuVol™ biosensor into cells and express them in large amounts.

Cell Types

Cortical Neurons

Application Notes

Applications:

Neurotoxicity testing for reporting cellular cationic fluctuations (such as calcium, protons/H+, potassium)
Phenotypic Assays
Chemical compound or small molecules screening
Research and development on animal models for human diseases
Studying neural maturation, networking and function, and cations-mediated pathways.

Species

Human

Properties

Size

~0.6x10^6 cells per 1ml of freezing medium (vial)

Growth Pattern

adherent

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.