![](static/images/product/product-icon-neural-cell-lines.png)
M5 Muscarinic Acetylcholine Receptor Assay Cell Line
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
DNA Accession Number: Gene Bank M80333
Official Full Name: Muscarinic acetylcholine receptor M5
Host Cell: DAG Maxensor U2OS Cell Line
Fluorescence: excitation/emission maxima at 574/602 nm
Resistances: Puromycin & G418
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Features
Application Notes
DAG Maxensor biosensor is a red fluorescent peptide that increases the fluorescence intensity in cells in the presence or absence of DAG. The increase in the concentration of the second messenger leads to changes in the folding of the Maxensor biosensor structure, thereby promoting its cell fluorescence intensity. In cell lines co-expressing DAG Maxensor biosensor and GPCR, after activating the receptor with its agonist, the activity of living cells can be easily quantified by fluorescence increase signal. The DAG Maxensor biosensor has true red fluorescence (excitation/emission maximum values of 574/602 nm respectively), which is most suitable for detection through the most popular filter sets and can be easily distinguished from background signals.
Research Areas
Assay
Assay Description
The M5 muscarinic acetylcholine receptor test involves the measurement of diacylglycerol (DAG) in the cytosol through PKC activation. The high reproducibility of this assay allows monitoring the activation of CHRM5 receptors in living cells.
Assay Characteristics
Agonist: Oxotremorine
EC50 Agonist: 1.48 x 106-8 M for DAG Flux
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry
Form
Cell Purity
Cell Viability
Mycoplasma Testing
Sterility Testing
Genetic Stability Testing
Shipping
Storage
Handling Advice
Research Use Only
Warnings
Quality Control
Target
Official Name
Full Name
Alternative Names
Publications (0)