Tel:
Fax:
Email:
Creative Biolabs

NeuroMab™ Anti-EPHA2 BBB Shuttle Antibody(NRZP-1022-ZP3851)

[CAT#: NRZP-1022-ZP3851]

Host Species:
Mouse
Species Reactivity:
Human
Applications:
WB; In Vitro; In Vivo; Inhib; Agonist

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

SPECIFIC INQUIRY

inquiry
Size:
Conjugation:
Endotoxin:
Purity:
Engineering:
Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

WB; In Vitro; In Vivo; Inhib; Agonist

Relevant Diseases

Glioblastoma Multiforme
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

EPHA2

Official Name

EPHA2

Full Name

EPH receptor A2 (ephrin type-A receptor 2)

Alternative Names

EPHA2; Epha2; AW545284; Eck; Myk2; Sek-2; Sek2; ARCC2; CTPA; CTPP1; CTRCT6; EPH receptor A2; ECK
Product Pictures
FuncS

Figure 1 EphA2 antibody inhibits malignant tumor cell growth in vitro. Purified EphA2 antibody was incubated with malignant and benign tumor15 cells in soft agar at 37°C for 7 days.

A549 malignant lung cancer cells were incubated with 10 μg/ml or 2.5 μg/ml of EA2 or EA5 monoclonal antibodies or control (PBS). All antibody amounts used inhibited cell growth in soft agar.

FuncS

Figure 2 EphA2 antibody EA2 inhibits tumor cell growth in vivo.

MDA-MB-231 breast cancer cells were implanted subcutaneously in athymic mice. After tumors grew to a mean volume of 30 to 100 mm3, mice were administered 6 mg/kg of the indicated antibody or negative control intraperitoneally twice weekly for 3 weeks. Total tumor volume was determined after sacrifice. Negative control is black and EA2 is white.

FuncS

Figure 3 EphA2 agonistic antibody EA2 reduces malignant growth.

1x105 control or MCF-7EPhA cells were suspended in soft agar in the presence or absence of tamoxifen (TAM, 1 pM) and EphA2 agonistic antibody (EA2, 10 μg/ml). Note that EA2 increased the sensitivity of MCF-7EIhA2 cells to tamoxifen.

FuncS

Figure 4 The EphA2 EA2 epitope is distinct from the ligand binding site.

EphA2-F was incubated with and bound to 5 immobilized Ephrin Al-Fe. Labeled EA2 was then incubated with the EphA2-ephrin A1 complex. Unlabeled competitors were incubated with the indicated amounts of EphA2-Ephrin A1-EA2 complexes. Competitors are Ephrin Al-Fc (black) or EA2 (white).

FuncS

Figure 5 Estrogen receptor expressed but functionally altered in 30 MCF-73EPA cells.

E2 indicates estrogen treatment; TAM indicates tamoxifen treatment; % conversion indicates the amount of substrate converted from non-acetylated substrate (non-AC) to acetylated substrate (AC) by 5 CAT enzyme.

Publications

Publications (0)

gift-card

Related Products
For Research Use Only. Not For Clinical Use.
Product
Hot Products
Fill out this form for a quote Inquiry Form Send Inquiry
webinar

The Spectrum of Stem Cell-Based Neuronal Models and Their Fit for Purpose

2:00 PM–3:00 PM EST, December 12, 2024

REGISTER NOW
Inquiry Basket
compare

Send inquiry