NeuroMab™ Anti-HMGB1 BBB Shuttle Antibody(NRZP-1022-ZP4010)

Figure 1: Representative incubations of 500 ng (lane 1) or 100 ng (lane 2) HMGB1 recombinant BoxA fragment (negative control) and 500 ng (lane 3) or 100 ng (lane 4) recombinant HMGB1 are shown. Western blot of anti-HMGB1 mAb DPH1.1 (upper panel) or control anti-Box-A subunit Ab Control (lower panel).

Figure 2: Representative photomicrographs showing mouse embryonic fibroblasts (MEFs) derived from wild-type (wt) mice or Hmgb1-/- mice immunofluorescently stained by anti-HMGB1 mAb DPH1.1. Scale bar represents 20 μm.

Figure 3: shows the migration of 3T3 cells towards HMGB1 in the presence of the indicated concentrations of anti-HMGB1 mAb, examined using a modified Boyden chamber assay. Each bar represents the mean number of migrated cells±standard deviation of triplicate samples.

Figure 4: Results of in vivo experiments showing the recruitment of inflammatory cells.

Figure 5A shows the results of representative immunohistochemical micrographs of control-treated livers from HBV transgenic mice two days after intravenous injection of 107 HBV-specific CTLs.

Figure 5B shows the results of representative immunohistochemical micrographs of Clo-L-treated livers from HBV transgenic mice two days after intravenous injection of 107 HBV-specific CTLs.

Figure 6A shows the results of in vivo experiments on sALT levels in blood.

Figure 6B shows the results of in vivo experiments on the absolute number of Gr-1high CD11b+ neutrophils (PMNs) recovered from the liver.