Dog High mobility group protein B1(HMGB1) ELISA kit

[CAT#: NRZP-0722-ZP268]

For the quantitative determination of dog high mobility group protein B1 (HMGB1) concentrations in serum, plasma, tissue homogenates, cell lysates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of dog HMGB1.

Species Reactivity

Dog

Marker

High-mobility group box 1

Properites

Sensitivity

In most cases, the sensitivity is below 0.078ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates, cell lysates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.312 ng/ml-20 ng/ml

Max Emission

450 nm

Target Details

Target

HMGB1

Official Name

HMGB1

Full Name

high mobility group box 1

Alternative Names

HMG1; HMG3; HMG-1; SBP-1

Gene ID

403170(Dog)

Uniprot ID

Q6YKA4(Dog)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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