NeuroMab™ Anti-HMGB1 BBB Shuttle Antibody(NRZP-1022-ZP4011)

Figure 1 shows the results of detection of human HMGB1 protein by Western blotting using human monoclonal antibodies.

"#127", "#129", "#194", "#459", "#130-008", "#213-012", "#213-001", "#283-010" in the picture , "#370-010" indicates the result of using a human monoclonal antibody as the primary antibody.

Figure 2 shows the results of surface plasmon resonance spectroscopy (SPR) detection of the interaction between a human monoclonal antibody (mAb #127) and human HMGB1 protein.

As a result of the analysis, the dissociation constant (KD) of monoclonal antibody #127 was 2.17×10-11 M, indicating that the antibody has extremely high affinity.

Figure 3 shows the detection results of the human MARCKS phosphorylation inhibitory activity of the human monoclonal antibody.

Figure 4 shows the effect of subcutaneous administration of the human monoclonal antibody (monoclonal antibody #129) on cognitive function and dendritic spine density in Alzheimer's disease model mice.

Fig. 1 shows the results of quantification of the cognitive function of each drug administration group by the Y-maze test.

Figure 5 shows the effect of subcutaneous administration of the human monoclonal antibody (monoclonal antibody #129) on cognitive function and dendritic spine density in Alzheimer's disease model mice.

Synapses (dendritic spines) revealed after imaging with two-photon excitation microscopy.

Figure 6 shows the effect of subcutaneous administration or intravenous injection of the human monoclonal antibody (monoclonal antibody #129) on the cognitive function of Alzheimer's disease model mice.

Fig. 1 shows the results of quantification of the cognitive function of each drug administration group by the Y-maze test. In the figure, "sc" represents subcutaneous injection, and "iv" represents intravenous injection. "B6/SJL" in the figure represents B6/SJL non-transgenic mice (normal mice), and "5xFAD" represents 5xFAD transgenic mice.

Figure 7 shows the localization of mAb #129 in the brain tissue of mice administered subcutaneously (sc injection) or intravenously (iv injection) of mAb #129. In the figure, "not injected" indicates the result of staining normal mouse brain without administration of antibody.