NeuroMab™ Anti-HMGB1 BBB Shuttle Antibody(NRZP-1022-ZP4009)

Figure 1 is a photograph showing the results of analysis of dendritic spines of an independent 5×FAD mouse group, etc., using a two-photon microscope.

Figure 2 is a graph showing the results of analysis of dendritic spines and the like in an independent AD model mouse group using a two-photon microscope.

Figure 3 is a photo of the DNA damage (γH2AX) detection results in the cerebral cortex of 6-month-old 5×FAD mice injected with anti-HMGB1 antibody subcutaneously at 1 to 6 months or using Western blot at 3 to 6 months of age.

Figure 4 is a graph showing the concentration of biotin-labeled IgG in plasma of mice and the like on day 3 after subcutaneous injection of IgG into mice.

Figure 5 is a photograph showing the results of analysis of the in vitro effects of HMGB1 and anti-HMGB1 antibodies on amyloid β (AM polymerization, Aβ oligomerization, Aβ-HMGB1 heteromer formation, and HMGB1 oligomerization), using anti-Aβ antibody Western blot antibodies.

Figure 6 is a photograph showing the results of observation of aggregated samples in vitro with an electron microscope.

Figure 7 is a graph showing the results of quantitative analysis of the number of microglial cells in the field of view (n=10, cerebral cortex) of the fluorescence micrographs.

Figure 8 is a graph showing the results of quantitative analysis of the number of plaques formed by microglia in the field of view (n=30, cerebral cortex) of the fluorescence micrographs.

Figure 9 is a graph showing the results of quantitative analysis of phagocytosis of fluorescent Aβ by primary cultured rat microglia.

Figure 10 is a photograph showing the results of in vitro polymerization analysis of HMGB1 in the absence of Aβ using an anti-HMGB1 antibody (rabbit-derived polyclonal antibody) by Western blotting.

Figure 11 is a photograph showing the results of in vitro polymerization of HMGB1 analyzed by Western blotting using an anti-HMGB1 antibody (rabbit-derived polyclonal antibody).

Figure 12 is a photograph showing the results of further analyzing the effect of HMGB1 and the anti-HMGB1 antibody on Aβ oligomerization by Western blotting using an anti-Aβ antibody (6E10).

Figure 13 is a photograph showing the results of immunohistochemical analysis in brain tissue using avidin-HRP and DAB chromogenicity to detect biotin-labeled IgG in mice subcutaneously injected with IgG.