NeuroMab™ Anti-HMGB1 Antibody(NRP-0422-P1369)

Figure 1 Binding kinetics and specificity of human anti-HMG1 antibodies.

HMG1 capture ELISA binding curves of several human anti-HMG1 antibodies demonstrate that these antibodies have different affinities for recombinant HMG1 produced in E. coli.

Figure 2 A number of human anti-HMG1 antibodies inhibited HMG1-stimulated cytokine release from human PBMCs.

Figure 3 Several human anti-HMG1 antibodies inhibit HMG1-stimulated cytokine gene expression in mouse macrophages (mMO).

Figure 4. A subset of human anti-HMG1 antibodies block the binding of recombinant HMG1 to RAGE.

Figure 5 Inhibitory effect of recombinant HMG1 on Thp-1 cells.

Representative dose response curves showing inhibition of recombinant HMG1 binding to Thp-1 cells by E11, G2 and a RAGE-Fc fusion protein are shown.

Figure 6 Human anti-HMG1 antibody is protective in a mouse model of acute lung injury (ALI).

Total cells recovered in BAL fluid from ALI mice treated with either G4 or E11 were reduced nearly 40% as compared to mice treated with either an isotype control antibody (R347). Treatment with an HMG1 A-box-Fc fusion protein only reduced total cells recovered by 23%.