Mouse Anti-HMGB1 Monoclonal Antibody (CBP2793)

Description

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.

Expression Host

Mammalian cells

Immunogen

HMGB1 fusion protein Ag1264

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2793

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Neurogenesis

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

HMGB1

Official Name

HMGB1

Full Name

High-mobility group box 1

Alternative Names

High mobility group box 1; High mobility group protein 1; High mobility group protein B1; HMG 1; HMG1; HMG3

Gene ID

3146(Human); 100862258(Mouse); 25459(Rat)

Uniprot ID

P09429(Human); P63158(Mouse); P63159(Rat)

1. Cui, H., Cheng, Y., He, Y., Cheng, W., Zhao, W., Zhao, H.,... & Cai, S. (2020). The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model. Molecular medicine reports, 22(5), 3723-3734. 2. Su, J., Pan, Y. W., Wang, S. Q., Li, X. Z., Huang, F., & Ma, S. P. (2020). Saikosaponin-d attenuated lipopolysaccharide-induced depressive-like behaviors via inhibiting microglia activation and neuroinflammation. International Immunopharmacology, 80, 106181. 3. Le, K., Daliv, E. C., Wu, S., Qian, F., Ali, A. I., Yu, D., & Guo, Y. (2019). SIRT1-regulated HMGB1 release is partially involved in TLR4 signal transduction: a possible anti-neuroinflammatory mechanism of resveratrol in neonatal hypoxic-ischemic brain injury. International immunopharmacology, 75, 105779.