NeuroMab™ Anti-OPN BBB Shuttle Antibody(NRZP-1022-ZP2618)

Fig.1 shows the results of the western blotting conducted on mOPN and rOPN.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.2 shows that the 39E1 antibody and 34E3 antibody inhibit adhesion of a murine melanoma cell strain to mOPN-5.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.3 shows that the 39E1 antibody and 34E3 antibody inhibit adhesion of a murine melanoma cell strain to mOPN-7.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.4 shows the results that the 39E1 antibody and 34E3 antibody recognize the polypeptides (I), (II) and hOPN N-half .

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.5 shows the results that the 39E1 antibody and 34E3 antibody do not react with hOPN but only with hOPN N-half.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.