NeuroMab™ Anti-OPN BBB Shuttle Antibody(NRZP-1022-ZP2596)

Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; WB; IHC; In Vitro; Neut

Relevant Diseases

Alzheimer's Disease; Neuroinflammation

Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only

Target

SPP1

Official Name

SPP1

Full Name

Secreted phosphoprotein 1

Alternative Names

BNSP;
Bone sialoprotein 1;
Bone sialoprotein I;
BSP I;
BSPI;
Early T lymphocyte activation 1;
ETA 1;
ETA1;
MGC110940;
Nephropontin;
OPN;
Osteopontin;

Product Pictures

SDS-PAGE

Fig.1 shows an SDS-PAGE analysis of purified MAbs against OPN using antibodies.

The fig shows MAb inhibition of cell adhesion to OPN.

ELISA

Fig.2 shows specificity differentiation testing of the anti-OPN monoclonal antibodies using an ELISA. Error bar+1 standard error of mean (SEM).

The fig shows MAb inhibition of cell adhesion to OPN.

ELISA

Fig.3 shows cross-reactivity of MAbs against human and mouse OPN.

The supernatants from the five monoclonal hybridoma (2E11, 2C5 2H9, 1F11 and 2F10) were probed against h-FL OPN and m-FL OPN in (5A) ELISA and in Western blot (5B=human probe and 5C=murine probe).

ELISA

Fig.4 shows the determination of epitope specifics on the anti-OPN MAbs by ELISA. Antibodies were probed against N-terminal and C-terminal fragments of OPN.

The supernatants from the five monoclonal hybridoma (2E11, 2C5 2H9, 1F11 and 2F10) were probed against h-FL OPN and m-FL OPN in (5A) ELISA and in Western blot (5B=human probe and 5C=murine probe).