NeuroMab™ Anti-SPP1 Antibody(NRP-0422-P688)

Figure 1 shows the EC50 determination of the four clones in the peptide antigen binding assay.

Data are shown as mean ± SE of 8 animals, 7 animals, and 5 animals in the control, 25 mg/kg, and 50 mg/kg groups, respectively. *p<0.05, **p<0.01: Significantly different from the control group according to Dunnet's multiple comparison test.

Figure 2 shows the EC50 values of the four clones binding to the OPN antigen peptide.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 3 shows the percent inhibition of human OPN-FL cleavage by four clones

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 4 shows the inhibition of thrombin cleavage of mouse OPN-FL by four clones determined by determining the production of a cleavage product, OPN-R, in an OPN-R-specific ELISA.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 5 shows the determination of EC50 values of four clones in a direct binding ELISA against mouse OPN-FL.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.