Mouse Anti-NES Monoclonal Antibody (CBP1515)

Description

Mouse anti-mouse nestin antibodies can recognize mouse nestin, a large intermediate filament protein transiently expressed in embryonic glial cells (Hockfield and McKay 1985). It is mainly expressed in stem cells of the developing nervous system. Terminal differentiation is related to the loss of nestin expression. In other embryonic tissues, the expression of nestin has also been noted in most glioblastoma polymorphs and many melanomas.

Immunogen

Nestin purified from embryonic rat spinal cord.

Species Reactivity

Rat; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1515

Applications

IF; IHC-P; IHC-Fr; WB

Application Notes

IHC-P (1/40-1/400); IHC-Fr (1/40-1/400)

Research Areas

Growth and Development

Conjugation

Unconjugated

Form

Liquid

Formulation

TRIS buffered glycine.
0.05% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

NES

Official Name

NES

Full Name

Nestin

Alternative Names

Nestin; Nbla00170

Gene ID

10763(Human); 18008(Mouse)

Uniprot ID

P48681(Human); Q6P5H2(Mouse); P21263(Rat)

1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system. 2. Arnold, T.D. et al. (2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β. 3. Mori, T. et al. (2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential. 4. Choi, J.S. et al. (2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina. 5. Choi, J.S. et al. (2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats. 6. Choi, J.S. et al. (2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord. 7. Shin, Y.J. et al. (2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats. 8. Zhang, H. et al. (2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors. 9. Aleksandrova, M.A. et al. (2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and Differentiation 10. Bertelli, E. et al. (2002) Nestin expression in rat adrenal gland. 11. Choi, J.S. et al. (2009) Enhanced expression of SOCS-2 in the rat hippocampus after transient forebrain ischemia. 12. Poltavtseva, R.A. et al. (2001) In vitro development of neural progenitor cells from human embryos. 13. Shin, Y.J. et al. (2013) Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats. 14. Barreira, A.L. et al. (2009) Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells after unilateral ureteral obstruction. 15. Araujo, M. R. et al. (2016) Mesenchymal stem cells promote augmented response of endogenous neural stem cells in spinal cord injury of rats 16. Shin, Y.J. et al. (2016) Increased expression of suppressor of cytokine signaling 2 in the subventricular zone after transient focal cerebral ischemia in adult rats.