Rat Anti-Mouse CD54 Monoclonal Antibody (CBP3745)

[CAT#: NAB2012124LS]

Anti-CD54 Monoclonal Antibody [Alexa Fluor 488 ]

Host Species:: Rat

Species Reactivity:: Mouse

Applications:: FC

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Immunogen

Mouse NS-1 cells

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2b, κ

Clone Number

CBP3745

Applications

Application Notes

FCM (≤0.25 µg per million cells in 100 µL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Alexa Fluor 594; Alexa Fluor 488; Alexa Fluor 498; Alexa Fluor 647; Alexa Fluor 700; APC; APC/Cyanine 7; APC/Fire 750; Biotin; Brilliant Violet 421; Brilliant Violet 510; Brilliant Violet 570; Brilliant Violet 570; Brilliant Violet 605; Brilliant Violet 650; Brilliant Violet 711; Brilliant Violet 750; Brilliant Violet 785; FITC; HRP; PE; Pacific Blue; PE/Cyanine 5; PE/Cyanine 7; PerCP; Spark YG 570

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD54

Official Name

ICAM1

Full Name

Intercellular Adhesion Molecule 1

Alternative Names

ICAM1; BB2; CD54; P3.58; intercellular adhesion molecule 1

Gene ID

3383 (Human); 15894 (Mouse)

Uniprot ID

P05362 (Human); P13597 (Mouse)

References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Publications