NeuroMab™ Anti-ICAM-1 BBB Shuttle Antibody(NRZP-1022-ZP2811)

Figure 1 Binding of seven unique scFv clones to Ramos cells (solid bars) and Jurkat cells (open bars). Control scFv (ctrl) did not bind to any cells.

Figure 2 Apoptosis induction by anti-Ramos scFv.

The seven unique scFv clones were incubated with Ramos B lymphoma cells at 37 C for 24 hours at various concentrations and the effect on apoptosis induction studied. Three scFv; Bl, B11, and C11, show titratable activity towards both cell lines, whereas apoptosis inducing capability

Figure 3 Specificities of isolated antibodies include HLA-DR/DP, IgM, and ICAM-1.

50-600 x 106 Raji B lymphoma cells were lysed with the non-ionic detergent Triton XlOOTM at 0.5 % v/v and immunoprecipitated with 100 g of the fully human IgG1 format of B1 (lane 1) and B11 (lane 2) antibody, followed by crosslinking with Protein A SepharoseTM. Ramos B lymphoma cell lysates, from 50 x 106 cells, were used for the precipitation of 20 ,g C11 (lane 3).

Figure 4 Specificities of isolated antibodies include HLA-DR/DP, IgM, and ICAM-1.

B1 IgG, B11 IgG, and Cll IgG binding to B lymphoma cells is specifically blocked by pre-incubation with anti-HLA-DR/DP, anti-ICAM-1 or anti-IgM antibodies, respectively.

Figure 5 Binding of Bl, B11, C11 IgGi to tumor cell lines from different sources. The antigen distribution of antigens targeted by B1, B11 and C11 antibodies on different cancer cell lines was studied by flow cytometry.