NK1 Tachykinin Receptor Assay Cell Line
[CAT#: NCL2110P264]
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: TACR1(NK1) /HEK293
Official Full Name: Tachykinin receptor 1
DNA Accesion Number: GenBank: AY462098
Host Cell: HEK293
Format: Cryopreserved vials
Resistance: Puromycin
Storage: Liquid Nitrogen
Official Full Name: Tachykinin receptor 1
DNA Accesion Number: GenBank: AY462098
Host Cell: HEK293
Format: Cryopreserved vials
Resistance: Puromycin
Storage: Liquid Nitrogen
Features
The HiTector TACR1 contains HEK293 cells stably expressing unlabeled human tachykinin receptor 1 (TACR1). The HiTector TACR1 cell line is designed to test compounds or analyze their ability to modulate tachykinin receptor 1. When the agonist binds to TACR1, the G protein is activated, which in turn triggers a cellular response mediated by the second messenger (calcium). This cell line has been validated by measuring the increase in calcium in the cytosol. The high reproducibility of this assay allows monitoring of TACR1 activation in high-throughput screening.
Cell Types
Other Cells
Research Areas
GPCR
Assay
NK1 Tachykinin Receptor Assay
Assay Description
Creative Biolabs' NK1 Tachykinin Receptor Assay (TACR1) allows compounds to be tested or analyzed for their ability to modulate TACR1. When the ligand binds to TACR1, it activates the G protein; this in turn triggers a calcium-mediated cellular response. The combination of agonist and TACR1 induces intracellular calcium mobilization and PKC activation through PLC-IP3 and DAG pathways, respectively.
Assay Characteristics
Readout: Calcium Flux
Agonist: Substance P
EC50 Agonist: 1.40 x 106-8 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Agonist: Substance P
EC50 Agonist: 1.40 x 106-8 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
TACR1
Full Name
Nk1 Tachykinin Receptor
Alternative Names
TACR1; NK1R; NKIR; SPR; TAC1R; tachykinin receptor 1
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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