pLenti-CMV-MCS-EF1α-PURO Lentiviral Expression Vector
- Applications:
- Virus Production
- Cell Types:
- Neural Lineage Cell
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Description
Lentiviral particles are produced from 293T cells by transient transfection of plasmids encoding viral particle components. For safety considerations regarding HIV-1 infectivity, recent lentiviral packaging systems have divided the viral components into 3 or 4 plasmids.
The pLenti-CMV-MCS-EF1α-PURO lentiviral expression vector contains the CMV promoter before multiple cloning sites (MCS) of the puromycin resistance gene driven by the EF1α promoter.
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