TSH Functional Assay Cell Line

[CAT#: NCL2110P315]

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

Cell line information: TSHR cAMP Maxensor cell line
Official Full Name: Thyroid-stimulating hormone receptor
DNA Accession Number: AY429111
Host Cell: HEK293
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The cAMP Maxensor TSHR contains HEK293 cells stably expressing cAMP Maxensor-FP650 biosensor and thyroid stimulating hormone receptor (without label). The Creative Biolabs cAMP Maxensor TSHR cell line is designed to test compounds or analyze their ability to modulate thyroid-stimulating hormone receptors. When the agonist binds to TSHR, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by analyzing the intracellular distribution of cAMP Maxensor biosensor by measuring the increase of cAMP in the cytosol. This cell line allows image analysis of compound-induced stimuli. The use of thyroid-stimulating hormone as an agonist validates this highly reproducible assay in high-content analysis (HCA) and high-throughput analysis (HTA).

Cell Types

Other Cells

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

TSH Functional Assay

Assay Description

Thyroid stimulating hormone (also called thyroid stimulating hormone, thyroid stimulating hormone, or TSH) is a pituitary hormone that stimulates the thyroid. To analyze the activity of rhTSH, we used a stable cell line that stably expresses the thyroid-stimulating hormone receptor (TSHR). This cell line also expresses the cAMP-Maxensor biosensor, which increases its fluorescence intensity after TSHR is activated. When rhTSH binds to TSHR, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (cAMP). The cAMP-Maxensor TSHR cell line measures the increase of cAMP in the cytoplasm in a dose-response manner in the presence of rhTSH.

Assay Characteristics

Readout: cAMP increase

Agonist: rhTSH

EC50 Agonist: 3.27 × 10⁶-8 gr/ml

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.