HSV-LSL-tdtomato-2a-TK(H356)

[CAT#: NTA-2011-ZP17] Review(5) Q&As(2)

Cre dependent, Anterograde, multisynaptic

Tracer Type:: Anterograde tracing virus

Applications:: Neural Tracing

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Product Overview

Description

Herpes simplex virus (HSV) based on the H129 strain has been widely used as an anterograde tracer. The addition of fluorescent protein-modified HSV can not only effectively mark the connections between different brain regions, but also the connections between the surrounding and the center.

Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.

Application
●Multi-synaptic tracking from the center of the brain to the periphery, or from lower to higher brain regions.
●Track the connection between peripheral organs and the central nervous system
●Track the neural network in the disease or injury model.
● Changes in neural networks during neural development.

Tracer Type

Anterograde tracing virus

Virus Type

Herpes Simplex Virus (HSV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde

Synaptic Restriction

Polysynaptic

Cell Types

Neuron

Expression

Normal Expression

Properites

Fluorophore

tdTomato

Storage

Please refer to the protocal.

Research Use Only

For research use only.

References

1. Bowers W J, Mastrangelo M A, Stanley H A, et al. HSV amplicon-mediated Abeta vaccination in Tg2576 mice:differential antigen-specific immune responses[J]. Neurobiol Aging, 2005,26(4):393-407.
2. Frazer M E, Hyghes J E, Mas trangelo M A, et al. Reduced pathology and improved behavioral performance in Alzheimer's disease mice vaccinated with HSV amplicons expressing amyloid-beta and interleukin-4[J]. Mol Ther, 2008, 16(5):845-853.
3. Coen, D.M., Kosz-Vnenchak, M., Jacobson, J.G., Leib, D.A., Bogard, C.L., Schaffer, P.A., Tyler, K.L., and Knipe, D.M. (1989). Thymidine kinase-negative herpes simplex virus mutants establish latency in mouse trigeminal ganglia but do not reactivate. Proceedings of the National Academy of Sciences of the United States of America 86, 4736-4740.
4. Li, X., Chen, W., Pan, K., Li, H., Pang, P., Guo, Y., Shu, S., Cai, Y., Pei, L., Liu, D., et al. (2018). Serotonin receptor 2c-expressing cells in the ventral CA1 control attention via innervation of the Edinger-Westphal nucleus. Nature neuroscience 21, 1239-1250.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Highly recommended for detailed neural tracing studies

The expression of the reporter gene was robust and consistent across different brain regions. The dual-reporter system allowed us to track both neural pathways and herpes simplex virus spread effectively. We were particularly impressed with the clarity of the fluorescence signal, which was bright and distinct, making our imaging analysis much easier.

Excellent

Our lab has been using various viral vectors for neural tracing

The tdtomato expression was very strong, and we observed minimal background noise. This product has significantly improved the quality of our neural mapping data, allowing us to identify previously unknown connections with great precision. The comprehensive user manual provided was also a great help.

Excellent

Overall, this product has exceeded my expectations

I used HSV-LSL-tdtomato-2a-TK(H356) for tracing long-range projections in the mouse brain. The tdtomato fluorescence was exceptionally bright, and the expression was specific to the targeted neurons. This made it easy to differentiate between traced and non-traced neurons. Additionally, the TK gene allowed for precise control over the viral spread, which was crucial for our experiments.

Excellent

This product has greatly enhanced the accuracy and reproducibility of our experiments

The tdtomato signal was consistently strong across multiple trials, and we encountered no issues with viral delivery. The combination of tdtomato and TK genes provides a versatile tool for both tracing and manipulating neural circuits.

Excellent

We are very pleased with its performance

The dual-reporter system allowed for detailed and accurate mapping of neural circuits. I also appreciated the technical support provided by the company, which was prompt and helpful. This product is a great investment for any neuroscience lab.

Q&As

How is the tdTomato fluorescence visualized, and what equipment is needed?

tdTomato fluorescence can be visualized using standard fluorescence microscopy equipment. The protein emits bright red fluorescence, making it easy to detect with a filter set optimized for red wavelengths (excitation: 554 nm, emission: 581 nm). Confocal or wide-field fluorescence microscopes with the appropriate filters will provide clear and detailed images of labeled neurons.

What are the expected transduction efficiency and expression levels in target neurons?

Transduction efficiency and expression levels can vary depending on factors such as target cell type, injection site, and viral titer. Generally, HSV vectors like HSV-LSL-tdTomato-2a-TK (H356) achieve high transduction efficiency in neurons, with robust expression of tdTomato visible within days post-injection. Optimize your experimental conditions to achieve the best results for your specific study.