Human CHO-K1/CB1/Gα15 Stable Cell Line

[CAT#: NCL20120037CR]

Cannabinoid Receptor 1 Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

The cannabinoid receptor CNR1 is Gs-coupled GPCRs expressed in primarily CNS and some peripheral neurones; particularly prevalent in basal ganglia, hippocampus, cerebellum, cerebral cortex and also presented in some non-neuronal cells and tissues, for example leukocytes and testis. A shorter human splice variant (411aa) has been identified in the brain and other tissues by reverse-transcriptase PCR but mRNA levels were less than 10-fold the levels of the longer isoform. The pharmacological characteristics of the isoforms are similar.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR; Neural Signal Transduction

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml HygromycinB, 200 μg/ml Zeocin

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

CNR1/CB1

Family

Cannabinoid receptor

Strain

CB1/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

CNR1

Official Name

CNR1

Alternative Names

Cannabinoid receptor; Brain-type cannabinoid receptor; Central cannabinoid receptor; Neuronal cannabinoid receptor; THC receptors; CNR; CB1K5; CB-R; CB1; CB1A; Cann6; SKR6R; cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB1R; Cann7

Gene ID

1268(Human); 12801(Mouse); 25248(Rat)

Uniprot ID

P21554(Human); P47746(Mouse); P20272(Rat)

References

1. Hamidi, S. R., Safdari, Y., & Arabi, M. S. (2019). Test bacterial inclusion body for activity prior to start denaturing and refolding processes to obtain active eukaryotic proteins. Protein expression and purification, 154, 147-151. 2. Banisadr, A., Safdari, Y., Kianmehr, A., & Pourafshar, M. (2018). Production of a germline-humanized cetuximab scFv and evaluation of its activity in recognizing EGFR-overexpressing cancer cells. Human vaccines & immunotherapeutics, 14(4), 856-863. 3. Pourafshar, M., Safdari, Y., Khajeniazi, S., Yazdani, Y., Banisadr, A., & Veisi, K. (2018). Production and Characterization of Single Chain Nimotuzumab: An In Vitro Study. International Journal of Peptide Research and Therapeutics, 24(1), 171-178.

Publications