Human SCNN1D Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP107]

Human SCNN1D knockout cell lysate

Species:: Human

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a HeLa cell transfected lysate in which Human SCNN1D has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Sodium Signaling

Marker

Sodium Channels

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

SCNN1D

Official Name

SCNN1D

Alternative Names

Sodium Channel Epithelial 1 Delta Subunit; Sodium Channel; Non-Voltage-Gated 1; Delta Subunit; Nonvoltage-Gated Sodium Channel 1 Subunit Delta; Sodium Channel; Nonvoltage-Gated 1; Delta; Epithelial Na(+) Channel Subunit Delta; Delta-ENaC; Delta-NaCH; ENaCd;