Product

Human TMED10 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP569]

Human TMED10 knockout cell lysate

Species:: Human

Cell Types:: HEK293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human TMED10 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Marker

Tangles & Tau

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

TMED10

Official Name

TMED10

Alternative Names

Transmembrane P24 Trafficking Protein 10; Transmembrane Protein Tmp21; P24 Family Protein Delta-1; S31III125; P24delta1; Tmp-21-I; P24delta; S31I125; TMP21; P23;

Publications