LHCGR Functional Assay Cell Line
[CAT#: NCL2110P316]
- Cell Types:
- Other Cells
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: cAMP- Maxensor LHCGR Cell Line
Cell Types
Other Cells
Research Areas
GPCR
Assay
LHCGR Functional Assay
Assay Description
The LHCGR functional assay allows measurement of the activity and potency of recombinant human LH (rhLH) and chorionic gonadotropin (rhCG). To analyze the activity of recombinant hormones, we used a stable cell line stably expressing LHCGR. This cell line also expresses the cAMP-Maxensor biosensor, which increases its fluorescence intensity when LHCGR is activated. When rhLH or rhCG binds to LHCGR, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (cAMP). This cAMP-Maxensor LHCGR cell line measures the increase of cAMP in the cytoplasm in a dose-response manner in the presence of any of the two recombinant hormones.
Assay Characteristics
Readout: cAMP increase
Agonist: hCG
EC50 Agonist: 5.76 × 106-3 IU/ml
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Agonist: hCG
EC50 Agonist: 5.76 × 106-3 IU/ml
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
LHCGR
Full Name
Lhcgr
Alternative Names
LHCGR; HHG; LCGR; LGR2; LH/CG-R; LH/CGR; LHR; LHRHR; LSH-R; ULG5; Luteinizing hormone/choriogonadotropin receptor
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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