NeuroMab™ Anti-SPP1 Antibody(NRP-0422-P699)

Figure 1 shows the results of western blotting for mOPN and rOPN.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 2 shows that 39E1 antibody and 34E3 antibody inhibit the adhesion of murine melanoma cell lines to mOPN-5.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 3 shows that 39E1 antibody and 34E3 antibody inhibit the adhesion of murine melanoma cell line to mOPN-7.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 4 shows the results of recognition of polypeptides (I), (II) and hOPN N-half by 39E1 antibody and 34E3 antibody.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.

Figure 5 shows the results that the 39E1 antibody and the 34E3 antibody did not react with hOPN but only reacted with hOPN N-half.

EC50 values were calculated from a 4 parameter fit equation using Prism Graphpad using the data from Figure 10. A2=2.6 ng/ml, A6=3.2 ng/ml; E5=4.0 ng/ml, G7=93.2 ng/ml.