NeuroMab™ Anti-SPP1 Antibody(NRP-0422-P679)

Figure 1 shows the results of detecting the binding ability of chimeric 2K1 antibody and humanized 2K1 antibody to hOPN5 peptide by ELISA method.

The figure shows inhibition of cell adhesion to OPN by MAbs.

Figure 2 shows the inhibitory effect of R2K1v1.7 and R2K1v0 on cell adhesion of human OPN.

The figure shows inhibition of cell adhesion to OPN by MAbs.

Figure 3 shows the effect of R2K1v1.7 on joint swelling in monkey collagen-induced arthritis.

Data are shown as mean ± SE of 8 animals, 7 animals, and 5 animals in the control, 25 mg/kg, and 50 mg/kg groups, respectively. *p<0.05, **p<0.01: Significantly different from the control group according to Dunnet's multiple comparison test.

Figure 4 shows the results of detecting the binding ability of purified R2K1v1.7-scFv to hOPN5 peptide by ELISA method.

Data are shown as mean ± SE of 8 animals, 7 animals, and 5 animals in the control, 25 mg/kg, and 50 mg/kg groups, respectively. *p<0.05, **p<0.01: Significantly different from the control group according to Dunnet's multiple comparison test.

Figure 5 is the SDS-PAGE results of F(ab')2 and purified F(ab')2-PEG of the intact molecular type R2K1v1.7 antibody and R2K1v1.7 antibody.

Data are shown as mean ± SE of 8 animals, 7 animals, and 5 animals in the control, 25 mg/kg, and 50 mg/kg groups, respectively. *p<0.05, **p<0.01: Significantly different from the control group according to Dunnet's multiple comparison test.

Figure 6 shows the results of detecting the binding ability of F(ab')2-PEG of R2K1v1.7 to hOPN5 peptide by BIAcore.

Data are shown as mean ± SE of 8 animals, 7 animals, and 5 animals in the control, 25 mg/kg, and 50 mg/kg groups, respectively. *p<0.05, **p<0.01: Significantly different from the control group according to Dunnet's multiple comparison test.