NeuroMab™ Anti-SPP1 Antibody(NRP-0422-P675)

Product Overview

Description

The antibody can be used for the detection of osteopontin fragments levels from the full-length osteopontin protein with disease detection, progression and prognosis.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; WB; IHC; In Vitro; Neut

Relevant Diseases

Alzheimer's Disease; Neuroinflammation

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

SPP1

Official Name

SPP1

Full Name

Secreted phosphoprotein 1

Alternative Names

BNSP;
Bone sialoprotein 1;
Bone sialoprotein I;
BSP I;
BSPI;
Early T lymphocyte activation 1;
ETA 1;
ETA1;
MGC110940;
Nephropontin;
OPN;
Osteopontin;

Gene ID

6696(Human)

Uniprot ID

P10451(Human)

Product Pictures

SDS-PAGE

Figure 1 shows the SDS-PAGE analysis of purified MAbs against OPN using antibodies.

The figure shows inhibition of cell adhesion to OPN by MAbs.

ELISA

Figure 2 shows the specificity discrimination test of anti-OPN monoclonal antibodies using ELISA. Error bars + 1 standard error of the mean (SEM).

The figure shows inhibition of cell adhesion to OPN by MAbs.

ELISA

Figure 3 shows the cross-reactivity of MAbs to human and mouse OPN.

Supernatants from five monoclonal hybridomas (2E11, 2C5, 2H9, 1F11 and 2F10) were detected against h-FL OPN and m-FL OPN for detection.

ELISA

Figure 4 shows the determination of the epitope specificity of anti-OPN MAbs by ELISA. Detection antibodies against the N- and C-terminal fragments of OPN.

Supernatants from five monoclonal hybridomas (2E11, 2C5, 2H9, 1F11 and 2F10) were detected against h-FL OPN and m-FL OPN for detection.