NeuroMab™ Anti-SPP1 Antibody(NRP-0422-P674)

Figure 1 shows the SDS-PAGE analysis of purified MAbs against OPN using antibodies.

Experimental results showed that cells treated with S-113-6 antibody had significantly lower cell migration ability than Mork group (PBS) and irrelevant antibody control group within 48 hours.

Figure 2 shows the specificity discrimination test of anti-OPN monoclonal antibodies using ELISA. Error bars + 1 standard error of the mean (SEM).

Experimental results showed that cells treated with S-113-6 antibody had significantly lower cell migration ability than Mork group (PBS) and irrelevant antibody control group within 48 hours.

Figure 3 shows the cross-reactivity of MAbs to human and mouse OPN.

Supernatants from five monoclonal hybridomas (2E11, 2C5, 2H9, 1F11 and 2F10) were detected against h-FL OPN and m-FL OPN for detection.

Figure 4 shows the determination of the epitope specificity of anti-OPN MAbs by ELISA. Detection antibodies against the N- and C-terminal fragments of OPN.

Supernatants from five monoclonal hybridomas (2E11, 2C5, 2H9, 1F11 and 2F10) were detected against h-FL OPN and m-FL OPN for detection.

Figure 5 shows the characterization of functionally neutralizing monoclonal antibodies against OPN-mediated cell adhesion.

The figure shows inhibition of cell adhesion to OPN by MAbs.