NeuroMab™ Mouse Anti-Rat Neuropilin 1 Monoclonal Antibody (CBP2224)

Product Overview

Description

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.

Immunogen

Extracellular region of mouse CD304

Species Reactivity

Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a, κ

Clone Number

CBP2224

Applications

Application Notes

FCM (≤0.25 µg per million cells in 100 µl volume)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1650

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.2 mg/ml

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD304

Official Name

NRP1

Full Name

Neuropilin-1

Alternative Names

NRP1; BDCA4; CD304; NP1; NRP; VEGF165R; Neuropilin 1

Gene ID

8829(Human); 18186(Mouse); 246331(Rat)

Uniprot ID

O14786(Human); P97333(Mouse); Q9QWJ9(Rat)

References

1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993.

Publications