pLenti-EF1-GFP-PGK-RFP-T2A-Puro Lentiviral Expression Vector (Reporter Plasmid)
- Applications:
- Virus Production
- Cell Types:
- Neural Lineage Cell
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Description
Lentiviral particles are produced from 293T cells by transient transfection of plasmids encoding viral particle components. For safety considerations regarding HIV-1 infectivity, recent lentiviral packaging systems have divided the viral components into 3 or 4 plasmids.
The pLenti-EF1-GFP-PGK-RFP-T2A-Puro lentivirus reporter gene plasmid contains the copGFP reporter gene, driven by the EF1α promoter, the tdTomato reporter gene and the puromycin resistance gene are driven by the PGK promoter.
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