Creative Biolabs

Brain Cortex Neurons [Rat]

[CAT#: NCC20-9PZ18]

Cryopreserved ampule of Rat Brain Cortex Neurons containing ≥4 million cells

Species:
Rat
Applications:
Cell Assay
Cell Types:
Neuron

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Description
Ready-to-use rat cortical neurons (E18, 19) are a cell suspension of high-quality primary embryonic brain neuron cells (including glial cells) prepared by standardized methods, which can be cultured immediately.

Each vial cortex cell contains approximately 4 million living cells. Using the recommended seeding density and medium, seed cells into 10-well or more wells of a 24-well plate and 50 wells or more of a 96-well plate. Cell death will occur within the first few days after seeding, and debris will be observed. this is normal. After about 4 days of culture, the cells will form a network of neurites, and by day 7, fragmentation will be minimized.

Recommended medium: CocKit™ Primary Neuron Growth Medium (NCC20-9PZ10)
Cell Types
Neuron
Applications
Cell Assay
Application Notes
• Guaranteed through 10 population doublings
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
Relevant Diseases
Alzheimer's Disease; Parkinson's Disease
Research Areas
Neurotoxicity; Neurogenesis
Species
Rat
Size
1 x Cryopreserved ampule of Rat Cortex Neurons containing ≥ 4,000,000 cells
Form
Cryopreserved
Shipping
Dry Ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased
For Research Use Only. Not For Clinical Use.
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