Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
[CAT#: NCC20-9PZ20]
Cryopreserved Rat Embryonic Dorsal Root Ganglion ampule containing ≥ 1,000,000 cells
- Species:
- Rat
- Applications:
- Cell Assay
- Cell Types:
- Neuron
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
The ready-to-use rat embryonic dorsal root ganglion (DRG) neurons are suspensions of high-quality sensory neurons prepared by standardized methods that can be cultured immediately.
Each vial of embryonic dorsal root ganglion neurons contains approximately 1 million cells in 0.25 ml of suspension. Some cell death will occur in the first few days after plating, and debris will be observed. this is normal. After about 4 days of culture, the cells will form a network of neurites, and by day 7, there will be very little debris. In the absence of mitotic inhibitors, neurons tend to aggregate (gangliolate) and detach from the matrix.
The staining of rat embryonic dorsal root ganglion neurons was Tuj positive. Primary neuronal cells need suitable substrates to adhere and survive. The preferred substrate is poly-D-lysine with laminin. Poly-D-lysine can also be used alone to coat cell culture plastic products or coverslips.
Tip: In order for embryonic DRG neurons to survive and grow, 100 ng/ml of nerve growth factor must always be added to the medium. In order to inhibit the proliferation of Schwann cells, it is also necessary to add a mitotic inhibitor.
Each vial of embryonic dorsal root ganglion neurons contains approximately 1 million cells in 0.25 ml of suspension. Some cell death will occur in the first few days after plating, and debris will be observed. this is normal. After about 4 days of culture, the cells will form a network of neurites, and by day 7, there will be very little debris. In the absence of mitotic inhibitors, neurons tend to aggregate (gangliolate) and detach from the matrix.
The staining of rat embryonic dorsal root ganglion neurons was Tuj positive. Primary neuronal cells need suitable substrates to adhere and survive. The preferred substrate is poly-D-lysine with laminin. Poly-D-lysine can also be used alone to coat cell culture plastic products or coverslips.
Tip: In order for embryonic DRG neurons to survive and grow, 100 ng/ml of nerve growth factor must always be added to the medium. In order to inhibit the proliferation of Schwann cells, it is also necessary to add a mitotic inhibitor.
Cell Types
Neuron
Applications
Cell Assay
Application Notes
• Guaranteed through 10 population doublings
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
Relevant Diseases
Alzheimer's Disease; Parkinson's Disease
Research Areas
Neurotoxicity; Neurogenesis
Species
Rat
Size
≥ 1,000,000 cryopreserved cells per ampule
Form
Cryopreserved
Shipping
Dry Ice
Storage
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
