Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons
Cryopreserved Rat Embryonic Dorsal Root Ganglion ampule containing ≥ 1,000,000 cells
Each vial of embryonic dorsal root ganglion neurons contains approximately 1 million cells in 0.25 ml of suspension. Some cell death will occur in the first few days after plating, and debris will be observed. this is normal. After about 4 days of culture, the cells will form a network of neurites, and by day 7, there will be very little debris. In the absence of mitotic inhibitors, neurons tend to aggregate (gangliolate) and detach from the matrix.
The staining of rat embryonic dorsal root ganglion neurons was Tuj positive. Primary neuronal cells need suitable substrates to adhere and survive. The preferred substrate is poly-D-lysine with laminin. Poly-D-lysine can also be used alone to coat cell culture plastic products or coverslips.
Tip: In order for embryonic DRG neurons to survive and grow, 100 ng/ml of nerve growth factor must always be added to the medium. In order to inhibit the proliferation of Schwann cells, it is also necessary to add a mitotic inhibitor.
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
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