Creative Biolabs

Brain Hypothalamus Neurons [Rat]

[CAT#: NCC20-9PZ58]

Cryopreserved ampule of Rat Hypothalamus Neurons containing ≥ 2 million cells

Cell Assay
Cell Types:

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The hypothalamus is responsible for many metabolic processes and other activities of the autonomic nervous system. It synthesizes and secretes neurohormones to stimulate or inhibit the secretion of pituitary hormones. The hypothalamus controls body temperature, hunger, thirst, fatigue and circadian rhythm. Rat hypothalamic neurons are an effective tool for studying neuroendocrine-related diseases.

Rat hypothalamic neurons were cryopreserved during the first passage of Sprague-Dawley rats of E18-19. They are positive for PGP and Tuj-1, but negative for infertility and mycoplasma. Each vial contains ≥ 2 million cells.

Recommended medium: CocKit™ Primary Neuron Growth Medium (NCC20-9PZ10)
Cell Types
Cell Assay
Application Notes
• Guaranteed through 10 population doublings
• Positive immunofluorescence staining for glial fibrillary acid protein (GFAP)
Relevant Diseases
Alzheimer's Disease; Parkinson's Disease
Research Areas
Neurotoxicity; Neurogenesis
≥ 2,000,000 cryopreserved cells per ampule
Dry Ice
Liquid nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
• A Certificate ofAnalysis is provided for each cell lot purchased
For Research Use Only. Not For Clinical Use.
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