ETA Endothelin Receptor Assay Cell Line
[CAT#: NCL2110P289]
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: HiTSeeker ETA (EDNRA)
Official Full Name: Endothelin receptor type A
DNA Accession Number: GenBank: AY275462
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen
Official Full Name: Endothelin receptor type A
DNA Accession Number: GenBank: AY275462
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen
Features
The HiTector EDNRA contains unlabeled U2OS cells that stably express Type A human endothelin receptor (ETA). Creative Biolabs' HiTector EDNRA (ETA) cell line is designed to test compounds or analyze their ability to modulate type A endothelin receptors.
Cell Types
Other Cells
Research Areas
GPCR
Assay
ETA Endothelin Receptor Assay
Assay Description
Creative Biolabs' ETA endothelin receptor test allows compounds to be tested or analyzed for their ability to modulate EDNRA. When the ligand binds to EDNRA, it activates the G protein, which in turn triggers a cellular response that contains calcium. Our EDNRA test uses Endothelin I as a positive control to measure changes in cytoplasmic calcium concentration. The high reproducibility of this assay allows monitoring of the EDNRA activation/inhibition process in high-throughput screening.
Assay Characteristics
Readout: Calcium Flux
Agonist: Endothelin I
EC50 Agonist: 5.04 x 106-10 M for Calcium Flux
Type of Assay: Cell-based/Functional
Detection Method: Fluorimetry
Agonist: Endothelin I
EC50 Agonist: 5.04 x 106-10 M for Calcium Flux
Type of Assay: Cell-based/Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
EDNRA
Full Name
Eta Endothelin Receptor
Alternative Names
EDNRA; ET-A; ETA; ETA-R; ETAR; ETRA; hET-AR; MFDA; endothelin receptor type A
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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