Human CHO-K1/ADORA3/Gα15 Stable Cell Line

[CAT#: NCL20120017CR]

Adenosine A3 Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

Extracellular adenosine mediates a multitude of biological effects, including wakefulness, antiarrythmia, bronchoconstriction and response to ischemia and oxidative stress. A family of four G-protein coupled adrenoceptors, A1, A2A, A2B and A3, is responsible for these effects. A3, which couples to Gi/o, is expressed in a wide range of human tissues, but most predominantly in the lung and liver. Recent animal model studies have shown that A3 receptors play important roles in brain ischemia, immunosuppresion, and bronchospasm. A3 receptor agonists and/or agonists may have important clinical value in the treatment of asthma and inflammation. Mice lacking A3 receptors display reduced mast cell degranulation and bronchoconstriction in response to adenosine.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 8 μg/ml Puromycin

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

ADORA3

Family

Adenosine receptor

Strain

ADORA3/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

A3AR

Official Name

A3AR

Full Name

Adenosine A3 receptor

Alternative Names

A3 receptor; adenosine A3 receptor; TGPCR1; ARA3; A3AR; A3R; AA3R

Gene ID

140(Human); 11542(Mouse); 100911796(Rat)

Uniprot ID

Q6P2N6(Human); Q61618(Mouse); P28647(Rat)

References

1. Zhang, W., Chen, L., Ma, Z., Du, L., & Li, M. (2014). Design, synthesis and biological evaluation of naphthalimidebased fluorescent probes for α1-adrenergic receptors. Drug Discoveries & Therapeutics, 8(1), 11-17.

Publications