Human DYRK1A Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP312]

Human DYRK1A knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human DYRK1A has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon12.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurogenesis

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

DYRK1A

Official Name

DYRK1A

Full Name

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A

Alternative Names

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A; Dual Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A; Dual Specificity YAK1-Related Kinase; Protein Kinase Minibrain Homolog; DYRK; MNBH; HP86; MNB; Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A; Dual Specificity Tyrosine-Phosphorylation-Regulated Kinase 1A;