Human HCN-2 Cells

[CAT#: NCB2108FY0005]

Human cells

Species:: Human

Applications:: 3D Cell Culture

Cell Types:: Neurons

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Product Overview

Description

Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Cell Types

Neurons

Applications

3D Cell Culture

Relevant Diseases

Encephalitis

Research Areas

Neural Cell

Species

Human

Expression Profile

tubulin; neurofilament protein; somatostatin; cholecystokinin-8

Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Neuronal

Culture Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 90%; fetal bovine serum, 10%

Freeze Medium

Complete growth medium supplemented with 5% (v/v) DMSO

Tissue Source

Brain

Age

7 weeks

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Research Use Only

For research use only

Donor Information

Gender

Female

Donor Health

Disease

Additional Donor Information

The HCN-2 cell line was derived from the cortical tissue of a patient who underwent hemispheric resection for intractable epilepsy associated with Rasmussen encephalitis.

Publications